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Goat Anti-HMHA1 / HA-1 Antibody (EB05126)

Code Name Applications Availability Size Tested species Price Grade  
EB05126 Goat Anti-HMHA1 / HA-1 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $255.00 Aspiring

Ordering Information for United States of America (change)

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Target Protein

Principal Names: HA-1, minor histocompatibility antigen HA-1, HLA-HA1, KIAA0223, HMHA1, histocompatibility (minor) HA-1
Official Symbol: ARHGAP45
Accession Number(s): NP_036424.2
Human GeneID(s): 23526
Important Comments: To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and won't recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353):1054-7, PMID: 9461441)

Immunogen

Peptide with sequence TLGSCRERQPEFV, from the C Terminus of the protein sequence according to NP_036424.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 65-70kDa band in Jurkat lysate at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 131kDda according to NP_036424.2.

Species Reactivity

Tested:
Expected from sequence similarity: Human