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Goat Anti-HOXD10 Antibody (EB08884)

Related Products: EBP08884 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08884 Goat Anti-HOXD10 Antibody Pep-ELISA, IHC, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08884 1
EB08884 (3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08884 2
EB08884 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: HOXD10, homeobox D10, HOX4, HOX4D, HOX4E, Hox-4.4, homeo box 4D, homeo box D10
Official Symbol: HOXD10
Accession Number(s): NP_002139.2
Human GeneID(s): 3236
Non-Human GeneID(s): 15430 (mouse), 303991 (rat)

Immunogen

Peptide with sequence PNRSCRIEQPVTQQ, from the internal region of the protein sequence according to NP_002139.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary testing showed a band at approx 37kDa in Human Kidney lysate after 3µg/ml antibody staining (calculated MW of 38.4 kDa according to NP_002139.2). Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Kidney shows nuclear staining in some epithelial cells of PCT. Recommended concentration, 3-5µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Horse, Cow

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