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Code Name Applications Availability Size Tested species Price Grade  
EB09834 Goat Anti-HOXD13 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human, Rat $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB09834 1
EB09834 (1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09834 2
EB09834 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09834 3
EB09834 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09834 4
EB09834 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09834 5
EB09834 Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: BDE, BDSD, homeo box 4I, homeo box D13, homeobox D13, HOX4I, SPD, HOXD13
Official Symbol: HOXD13
Accession Number(s): NP_000514.2
Human GeneID(s): 3239
Non-Human GeneID(s): 15433 (mouse), 288154 (rat)

Immunogen

Peptide with sequence C-HGVGLQQNALKSSPH, from the internal region of the protein sequence according to NP_000514.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 37kDa band observed in Rat Brain lysates (calculated MW of 34.9kDa according to NP_001099356.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 8µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nucleus of HEK293 and U2OS cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig

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