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Goat Anti-HPK1 / MAP4K1 Antibody - Trial (EB05454-T)

Related Products: EBP05454 - Immunizing Peptide, Goat Anti-HPK1 / MAP4K1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05454-T Goat Anti-HPK1 / MAP4K1 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05454-T 1
EB05454 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05454-T 2
EB05454 (2.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MAP4K1, mitogen-activated protein kinase kinase kinase kinase 1, HPK1, hematopoietic progenitor kinase 1
Official Symbol: MAP4K1
Accession Number(s): NP_001036065.1; NP_009112.1
Human GeneID(s): 11184
Important Comments: This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).

Immunogen

Peptide with sequence DVVDPDIFNRDPR-C, from the N Terminus of the protein sequence according to NP_001036065.1; NP_009112.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2µg/ml.
IHC: Paraffin embedded Human Thymus. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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