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Goat Anti-HSPA13 / STCH (aa83-95) Antibody (EB11978)

Code Name Applications Availability Size Tested species Price Grade  
EB11978 Goat Anti-HSPA13 / STCH (aa83-95) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB11978 1
EB11978 (0.1µg/ml) staining of Human Uterus lysate (A) + peptide (B) and (0.3ug/ml) Colon lysate (C) + peptide (D) (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HSPA13, heat shock protein 70kDa family, member 13, STCH, heat shock 70 kDa protein 13, microsomal stress 70 protein ATPase core, microsomal stress-70 protein ATPase core, stress 70 protein chaperone, microsome-associated, 60kD, stress 70 protein chaperone, microsome-associated, 60kDa, stress-70 protein chaperone microsome-associated 60 kDa protein
Official Symbol: HSPA13
Accession Number(s): NP_008879.3
Human GeneID(s): 6782

Immunogen

Peptide with sequence C-ESVELADSNPQNT, from the internal region of the protein sequence according to NP_008879.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60-65kDa band observed in Human Uterus and Colon lysates (calculated MW of 51.9kDa according to NP_008879.3)..This band was successfully blocked by incubation with the immunizing peptide and corresponds to different antibodies from other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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