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Goat Anti-HSPA5 / GRP78 (aa118-131) Antibody (EB12856)

Code Name Applications Availability Size Tested species Price Grade  
EB12856 Goat Anti-HSPA5 / GRP78 (aa118-131) Antibody Pep-ELISA, IF, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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ARP American Research Products, Inc
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EB12856 1
EB12856 (0.1µg/ml) staining of HepG2 (A), HeLa (B) and MCF7 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12856 2
EB12856 (0.01µg/ml) staining of Mouse Liver (A) and Testes (B) and Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12856 3
EB12856 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: HSPA5, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), BIP, GRP78, HEL-S-89n, MIF2, 78 kDa glucose-regulated protein, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, epididymis secretory sperm binding protein Li 89n, immunoglobu
Official Symbol: HSPA5
Accession Number(s): NP_005338.1
Human GeneID(s): 3309


Peptide with sequence C-KVVEKKTKPYIQVD, from the internal region of the protein sequence according to NP_005338.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 75kDa band observed in lysates of cell lines HepG2, HeLa and MCF7, and in Mouse Liver and Testis lysates, and approx. 80kDa band in Rat Brain lysates (calculated MW of 72.3kDa according to Human NP_005338.1 and Rat NP_037215.1, and 72.4kDa according to Mouse NP_071705.3). This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-0.1µg/ml. Primary incubation was 1 hour.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human

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