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Goat Anti-HTR7 / 5-HT7 Antibody (EB09347)

Related Products: EBP09347 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09347 Goat Anti-HTR7 / 5-HT7 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09347 1
EB09347 (1µg/ml) staining of KELLY lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HTR7, 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled), 5-HT7, 5-hydroxytryptamine receptor 7, OTTHUMP00000020077, OTTHUMP00000020078, OTTHUMP00000020079, serotonin 5-HT-7 receptor
Official Symbol: HTR7
Accession Number(s): NP_000863.1
Human GeneID(s): 3363
Important Comments: This antibody is expected to recognize isoform a (NP_000863.1) only.

Immunogen

Peptide with sequence QNADYCRKKGHDS, from the C Terminus of the protein sequence according to NP_000863.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50kDa band observed in lysates of cell line KELLY (calculated MW of 49.5kDa according to NP_000863.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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