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Code Name Applications Availability Size Tested species Price Grade  
EB11801 Goat Anti-IDE (long isoform aa10-14)) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB11801 1
EB11801 (1µg/ml) staining of HEK293 (A) and HeLa (B) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Abeta-degrading protease, IDE, insulin protease, insulinase, insulin-degrading enzyme, INSULYSIN
Official Symbol: IDE
Accession Number(s): NP_004960.2; NP_001159418.1
Human GeneID(s): 3416

Immunogen

Peptide with sequence HPALPSTFRSVLGAR-C, from the N Terminus of the protein sequence according to NP_004960.2; NP_001159418.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 58-60kDa band observed in cell lysates of HEK293 and HeLa calculated MW of 54.2kDa according to NP_001159418.1). Recommended concentration: 1-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

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