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Goat Anti-IDH2 Antibody (EB10529)

Related Products: EBP10529 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10529 Goat Anti-IDH2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10529 1
EB10529 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10529 2
EB10529 (0.1µg/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10529 3
EB10529 (3.75µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ICD-M, IDH, IDHM, IDP, IDPM, isocitrate dehydrogenase 2 (NADP+), mitochondrial, isocitrate dehydrogenase, mitochondrial, mNADP-IDH, NADP+-specific ICDH, oxalosuccinate decarboxylase, IDH2
Official Symbol: IDH2
Accession Number(s): NP_002159.2
Human GeneID(s): 3418
Non-Human GeneID(s): 269951 (mouse), 361596 (rat)

Immunogen

Peptide with sequence CIHGLSNVKLNE, from the internal region of the protein sequence according to NP_002159.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 48kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 50.9kDa according to Mouse NP_766599.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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