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Goat Anti-IFT88 / Polaris Antibody (EB07088)

Related Products: EBP07088 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07088 Goat Anti-IFT88 / Polaris Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07088 1
EB07088 (2µg/ml) staining of K562 (A), and (3ug/ml) THP-1 (B), U251 (C) and KNRK (D) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07088 2
EB07088 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07088 3
EB07088 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar transport 88 homolog, recessive polycystic kidney disease protein Tg737 homolog, intraflagellar transport 88 homolog (Chlamydomonas)
Official Symbol: IFT88
Accession Number(s): NP_783195.2; NP_006522.2, NP_001340507.1; NP_001340498.1
Human GeneID(s): 8100
Non-Human GeneID(s): 21821 (mouse), 305918 (rat)
Important Comments: This antibody is expected to recognise many reported isoforms.

Immunogen

Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2; NP_006522.2, NP_001340507.1; NP_001340498.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 80kDa band observed in lysates of cell lines THP-1, K562 and KNRK and 85kDa in lysates of cell line U251 (calculated MW of 94.3kDa according to Human NP_783195.2) and 93.1kDa according to Rat XP_006252128.1) . These bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.


Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 and HepG2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody (previous batch) has been successfully used in IF on Mouse:
Miyoshi K, Kasahara K, Miyazaki I, Shimizu S, Taniguchi M, Matsuzaki S, Tohyama M, Asanuma M.
Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice.
FASEB J. 2009 Oct;23(10):3289-97.
PMID: 19470799

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