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|EB07088||Goat Anti-IFT88 / Polaris Antibody||Pep-ELISA, WB, IHC, IF, FC||100µg specific antibody in 200µl||Human, Mouse||$315.00|
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Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2; NP_006522.2, NP_001340507.1; NP_001340498.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: Expression of the protein seen within the nucleus and cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml. This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells as described in the following paper: Miyoshi et al, FASEB J. 2009 Oct;23(10):3289-97, PMID: 19470799.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
This antibody has been successfully used in IF on Mouse:
Miyoshi K, Kasahara K, Miyazaki I, Shimizu S, Taniguchi M, Matsuzaki S, Tohyama M, Asanuma M.
Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice.
FASEB J. 2009 Oct;23(10):3289-97.