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Goat Anti-IGF2BP2 Antibody (EB08289)

Related Products: EBP08289 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08289 Goat Anti-IGF2BP2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08289 1
EB08289 (1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08289 2
HEK293 overexpressing IGF2BP2 (RC205673) and probed with EB08289 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: IGF2BP2, insulin-like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, VICKZ2, p62, IGF II mRNA binding protein 2, IGF-II mRNA-binding protein 2
Official Symbol: IGF2BP2
Accession Number(s): NP_006539.3; NP_001007226.1
Human GeneID(s): 10644
Non-Human GeneID(s): 319765 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_006539.3 and NP_001007226.1).

Immunogen

Peptide with sequence C-KQQEQKYPQGVASQR, from the C Terminus of the protein sequence according to NP_006539.3; NP_001007226.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

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