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Goat Anti-IKAROS / IKZF1 Antibody (EB09708)

Related Products: EBP07847 - Immunizing Peptide, EBP09708 - Immunizing Peptide, Goat Anti-IKZF1 / IKAROS Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09708 Goat Anti-IKAROS / IKZF1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09708 1
EB09708 (3.8µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: IKZF1, IKAROS family zinc finger 1 (Ikaros), Hs.54452, IK1, IKAROS, LYF1, PRO0758, ZNFN1A1, hIk-1, CLL-associated antigen KW-6, Ikaros (zinc finger protein), zinc finger protein, subfamily 1A, 1 (Ikaros), lymphoid transcription factor LyF-1
Official Symbol: IKZF1
Accession Number(s): NP_006051.1; NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1
Human GeneID(s): 10320
Important Comments: This antibody is expected to recognise all isoforms NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1

Immunogen

Peptide with sequence C-DTPYDSSASYEKEN, from the internal region of the protein sequence according to NP_006051.1; NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Preliminary testing showed a band at approx. 60kDa in Human Lymph node and Peripheral Blood Monocyte lysate, after 1ug/ml antibody staining (calculated MW of 57.5kDa according to NP_006051.1). Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Tonsil shows textured staining in some nuclei of the lymphoid tissue. Recommended concentration: 2.5-3.8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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