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Goat Anti-IKZF4 Antibody (EB09810)

Related Products: EBP09810 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09810 Goat Anti-IKZF4 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human, Mouse $464.00 Elite_Plus

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EB09810 1
EB09810 (1ug/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09810 2
EB09810 (2µg/ml) staining of Jurkat nuclear lysate (A) + Peptide (B), and (0.1ug/ml) staining of K562 nuclear lysate (C) + Peptide (D), (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09810 3
EB09810 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09810 4
EB09810 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09810 5
EB09810 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Target Protein

Principal Names: EOS, IKAROS family zinc finger 4 (Eos), KIAA1782, zinc finger protein, subfamily 1A, 4, zinc finger protein, subfamily 1A, 4 (Eos), zinc finger transcription factor Eos, ZNFN1A4, IKZF4
Official Symbol: IKZF4
Accession Number(s): NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1
Human GeneID(s): 64375
Non-Human GeneID(s): 22781 (mouse)
Important Comments: This antibody is expected to recognise reported isoforms NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1

Immunogen

Peptide with sequence C-RPTFIDRLANSLTKR, from the internal region of the protein sequence according to NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 60kDa band observed in nuclear lysates of cell line Jurkat, approx.. 60+70kDa in nuclear lysates of cell line K562, and approx. 55kDa in Mouse Heart lysates (calculated MW of 64.1kDa according to Human NP_071910.3, 59.1kDa Human NP_001338019.1, and 58.1kDa according to Mouse NP_001345465.1). All bands were successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-2µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: (5)µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog, Cow