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Goat Anti-IRAK3 (mouse) Antibody - Trial (EB08674-T)

Related Products: EBP08674 - Immunizing Peptide, Goat Anti-IRAK3 (mouse) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08674-T Goat Anti-IRAK3 (mouse) Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Mouse, Rat $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB08674-T 1
B08674 (0.1ug/ml) staining of Rat Liver lysate (35μg protein in RIPA buffer). Detected with chemiluminescence.
EB08674-T 2
EB08674 (0.5μg/ml) staining of Mouse Bone Marrow (wildtype first lane, KO second lane) lysates (35μg protein in RIPA buffer). Primary incubation was overnight at 4C Detected with chemiluminescence. Data obtained from anonymous customer.
Click here to download PDF datasheet.

Target Protein

Principal Names: IRAK3, interleukin-1 receptor-associated kinase 3, ASRT5, IRAK-M, IRAKM, interleukin-1 receptor-associated kinase M
Official Symbol: IRAK3
Accession Number(s): NP_082955.2
Non-Human GeneID(s): 73914 (mouse), 314870 (rat)

Immunogen

Peptide with sequence C-GHSYGSKPMEKR, from the internal region (near the C Terminus) of the protein sequence according to NP_082955.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 65-70kDa band observed in Rat Liver lysates, and in preliminary testing of Rat Adrenal Gland lysate (calculated MW of 69.0kDa according to Rat, NP_001101571.1 and 67.8kDa according to Mouse NP_082955.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.

A customer detected a band at approx. 70kDa in Mouse Bone Marrow lysates, which was not present in the knock-out mouse.


Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat

Specific Reference

This antibody has been successfully used in the following paper:
Gurcan Tunali, Marta Rúbies Bedós, Divya Nagarajan, Patrik Fridh, Irineos Papakyriacou, Yumeng Mao
IL-1 receptor-associated kinase-3 acts as an immune checkpoint in myeloid cells to limit cancer immunotherapy
J Clin Invest. 2023 Apr 3;133(7):e161084.
PMID: 36757800

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