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Goat Anti-IRAK3 (mouse) Antibody - Trial (EB08674-T)

Code Name Applications Availability Size Tested species Price Grade  
EB08674-T Goat Anti-IRAK3 (mouse) Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Mouse, Rat $105.00 Elite

Ordering Information for

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
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EB08674-T 1
EB08674 (0.05μg/ml) staining of Rat Liver lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected with chemiluminescence.
EB08674-T 2
EB08674 (0.5μg/ml) staining of Mouse Bone Marrow (wildtype first lane, KO second lane) lysates (35μg protein in RIPA buffer). Primary incubation was overnight at 4C Detected with chemiluminescence. Data obtained from anonymous customer
Click here to download PDF datasheet.

Target Protein

Principal Names: IRAK3, interleukin-1 receptor-associated kinase 3, ASRT5, IRAK-M, IRAKM, interleukin-1 receptor-associated kinase M
Official Symbol: IRAK3
Accession Number(s): NP_082955.2
Non-Human GeneID(s): 73914 (mouse), 314870 (rat)


Peptide with sequence C-GHSYGSKPMEKR, from the internal region (near the C Terminus) of the protein sequence according to NP_082955.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 65kDa band observed in Rat Liver lysates (calculated MW of 67.8kDa according to NP_082955.2). An approx 70kDa is observed in Mouse Bone Marrow lysates but this band disappears in lysates from the knock-out mouse. Recommended concentration: 0.05-0.2µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat

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