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Goat Anti-IRF2 Antibody (EB05582)

Related Products: EBP05582 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05582 Goat Anti-IRF2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05582 1
EB05582 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: IRF2, interferon regulatory factor 2, DKFZp686F0244, IRF-2
Official Symbol: IRF2
Accession Number(s): NP_002190.2
Human GeneID(s): 3660
Non-Human GeneID(s): 16363 (mouse)

Immunogen

Peptide with sequence KTSDITQARVKSC, from the C Terminus of the protein sequence according to NP_002190.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 48kDa band observed in lysates of cell line Jurkat (calculated MW of 39.3kDa according to NP_002190.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al, Invest Ophthalmol Vis Sci. 1999 Apr;40(5):976-82.; PMID: 10102295. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Liljeroos M, Vuolteenaho R, Rounioja S, Henriques-Normark B, Hallman M, Ojaniemi M.
Bacterial ligand of TLR2 signals Stat activation via induction of IRF1/2 and interferon-alpha production.
Cell Signal. 2008 Oct;20(10):1873-81.
PMID: 18652890

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