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Goat Anti-IRF2BP1 (aa461-474) Antibody (EB10698)

Code Name Applications Availability Size Tested species Price Grade  
EB10698 Goat Anti-IRF2BP1 (aa461-474) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB10698 1
EB10698 (1µg/ml) staining of nuclear NIH3T3 lysate (A) and of Mouse (B) and Rat (C) Colon lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10698 2
EB10698 (0.5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: IRF2BP1, interferon regulatory factor 2 binding protein 1, DKFZp434M154
Official Symbol: IRF2BP1
Accession Number(s): NP_056464.1
Human GeneID(s): 26145

Immunogen

Peptide with sequence C-QPPTQHRLVARNGE, from the internal region of the protein sequence according to NP_056464.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.7kDa according to NP_056464.1). An approx 85kDa band observed in nuclear lysates of cell line NIH3T3 and 75kDa in Mouse and Rat Colion lysates. This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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