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Goat Anti-ITIH4 (aa890-903) Antibody (EB10921)

Related Products: EBP09084 - Immunizing Peptide, EBP10921 - Immunizing Peptide, Goat Anti-ITIH4 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10921 Goat Anti-ITIH4 (aa890-903) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10921 1
EB10921 (0.03µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10921 2
EB10921 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ITIH4, inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein), DKFZp686G21125, H4P, IHRP, ITIHL1, PK120, ITI heavy chain H4, ITI-HC4, OTTHUMP00000213834, OTTHUMP00000213869, PK-120, gp120, inter-alpha-inhibitor
Official Symbol: ITIH4
Accession Number(s): NP_002209.2; NP_001159921.1
Human GeneID(s): 3700
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002209.2; NP_001159921.1).

Immunogen

Peptide with sequence C-SDDGRRTLRVQGND, from the C Terminus of the protein sequence according to NP_002209.2; NP_001159921.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 150kDa band observed in Human Placenta lysates (calculated MW of 103kDa according to NP_002209.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis (Leydig cells). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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