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Goat Anti-JAM2 / JAMB / CD322 Antibody (EB09490)

Related Products: EBP09140 - Immunizing Peptide, EBP09490 - Immunizing Peptide, Goat Anti-JAM2 / JAM-B Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09490 Goat Anti-JAM2 / JAMB / CD322 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB09490 1
EB09490 (0.5µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: JAM2, junctional adhesion molecule 2, C21orf43, CD322, JAM-B, JAMB, PRO245, VE-JAM, VEJAM, JAM-IT/VE-JAM, OTTHUMP00000096100, junctional adhesion molecule B, vascular endothelial junction-associated molecule
Official Symbol: JAM2
Accession Number(s): NP_067042.1
Human GeneID(s): 58494
Non-Human GeneID(s): 67374 (mouse), 619374 (rat)

Immunogen

Peptide with sequence C-QRKGYFSKETSFQ, from the internal region of the protein sequence according to NP_067042.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 35kDa band observed in Human Heart lysates (calculated MW of 33.2kDa according to NP_067042.1). Recommended concentration: 0.5-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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