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Code Name Applications Availability Size Tested species Price Grade  
EB08090 Goat Anti-KCC3 / SLC12A6 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08090 1
EB08090 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08090 2
EB08090 (2.5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SLC12A6, KCC3, solute carrier family 12 (potassium/chloride transporters), member 6, ACCPN, DKFZP434D2135, KCC3A, KCC3B, potassium chloride cotransporter 3, electroneutral potassium-chloride cotransporter 3, K-Cl cotransporter 3, potassium chloride cotransporter KCC3a-S3, potassium-chloride transporter-3a, potassium-chloride transporter-3b, solute carrier family 12 (potassium/chloride transporter), member 6
Official Symbol: SLC12A6
Accession Number(s): NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1; NP_005126.1; NP_598408.1
Human GeneID(s): 9990
Important Comments: This antibody is expected to recognise all reported isofoms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035961.1; NP_001035962.1); Reported variants represent identicial protein: NP_001035959.1; NP_001035960.1

Immunogen

Peptide with sequence SRHIDVCSKTKE, from the internal region of the protein sequence according to NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1; NP_005126.1; NP_598408.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 120kDa band observed in Human Heart and Kidney lysates (calculated MW ranging 121-128kDa according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Heart shows staining of myocardial fibrils in transverse section. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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