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Goat Anti-KCNN2 Antibody (EB09678)

Related Products: EBP09678 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09678 Goat Anti-KCNN2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09678 1
EB09678 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09678 2
EB09678 (3.75µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09678 3
EB09678 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: KCNN2, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2, KCa2.2, SK2, SKCA2, hSK2, apamin-sensitive small-conductance Ca2+-activated potassium channel, small conductance calcium-activated potassium channel protein 2
Official Symbol: KCNN2
Accession Number(s): NP_067627.2; NP_740721.1
Human GeneID(s): 3781
Non-Human GeneID(s): 140492 (mouse), 54262 (rat)
Important Comments: This antibody is expected to recognize both isoforms (NP_067627.2; NP_740721.1).

Immunogen

Peptide with sequence C-ESYDKHVTYNAER, from the internal region of the protein sequence according to NP_067627.2; NP_740721.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 70kDa and 23kDa bands observed in Human Liver lysates (calculated MW of 63.8kDa according to NP_067627.2 and of 26.3kDa according to NP_740721.1). Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Thyroid Gland and Kidney. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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