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Goat Anti-KEAP1 (aa41-53) Antibody (EB10963)

Code Name Applications Availability Size Tested species Price Grade  
EB10963 Goat Anti-KEAP1 (aa41-53) Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human, Mouse $333.00 Elite

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EB10963 1
EB10963 (0.2µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer).. Detected by chemiluminescence.
EB10963 2
EB10963 (3.8µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB10963 3
EB10963-P1 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: KEAP1, kelch-like ECH-associated protein 1, INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, cytosolic inhibitor of Nrf2, kelch-like protein 19
Official Symbol: KEAP1
Accession Number(s): NP_036421.2
Human GeneID(s): 9817
Non-Human GeneID(s): 50868 (mouse), 117519 (rat)
Important Comments: Reported variants represent identical protein: NP_036421.2, NP_987096.1


Peptide with sequence C-EVTPSQHGNRTFS, from the internal region (near N Terminus) of the protein sequence according to NP_036421.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kDa according to Human NP_036421.2 and of 69.6kDa according to Mouse NP_057888.1). Recommended concentration: 0.2-0.6µg/ml.
IHC: In paraffin embedded Human Cerebellum shows textured cytoplasm staining in Purkinje cell and processes. Recommended concentration, 3-5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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