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Goat Anti-KLF15 Antibody (EB05463)

Related Products: EBP05463 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05463 Goat Anti-KLF15 Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05463 1
EB05463 (1µg/ml) staining of nuclear HepG2 (A) and (0.5ug/ml) HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05463 2
EB05463 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: KLF15, KKLF, Kruppel-like factor 15, KKLF protein, kidney-enriched Kruppel-like factor, DKFZp779M1320
Official Symbol: KLF15
Accession Number(s): NP_054798.1
Human GeneID(s): 28999
Non-Human GeneID(s): 66277 (mouse), 85497 (rat)

Immunogen

Peptide with sequence VDHLLPVDENFSS-C, from the N Terminus of the protein sequence according to NP_054798.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 45kDa band observed in HeLa and in nuclear cell lysates of HepG2 (calculated MW of 44kDa according to NP_054798.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat, Mouse and Pig Kidney, and NIH3T3 for this particular batch.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig

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