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Goat Anti-KRT13 Antibody (EB09037)

Related Products: EBP09037 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09037 Goat Anti-KRT13 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09037 1
EB09037 (0.1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09037 2
EB09037 (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: KRT13, keratin 13, CK13, K13, MGC161462, MGC3781, cytokeratin 13, keratin, type I cytoskeletal 13
Official Symbol: KRT13
Accession Number(s): NP_705694.2
Human GeneID(s): 3860
Important Comments: This antibody is expected to recognize the reported isoform a (NP_002265.2) only.

Immunogen

Peptide with sequence C-TSNASGRRTSDVRRP, from the C Terminus of the protein sequence according to NP_705694.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 55kDa band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6kDa according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr;149(Pt 4):961-71.; PMID: 12686638). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Tonsil shows strong layered, filamentous staining. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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