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Goat Anti-Ku80 / XRCC5 Antibody - Trial (EB07209-T)

Related Products: EBP07209 - Immunizing Peptide, Goat Anti-Ku80 / XRCC5 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07209-T Goat Anti-Ku80 / XRCC5 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB07209-T 1
EB07209 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07209-T 2
EB07209 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: XRCC5, KU80, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining Ku autoantigen, 80kDa), HGNC:12833, KARP-1, KARP1, Ku86, NFIV, ATP-dependent DNA helicase II, DNA repair protein XRCC5, Ku86 autoantigen related protein 1, Ku autoantigen, 80kD, X-ray repair, complementing defective, repair in Chinese hamster
Official Symbol: XRCC5
Accession Number(s): NP_066964.1
Human GeneID(s): 7520

Immunogen

Peptide with sequence C-SLAKKDEKTDTLED, from the internal region of the protein sequence according to NP_066964.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 80-85kDa band observed in lysates of HeLa (calculated MW of 82.7kDa according to NP_066964.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of 75kDa was observed consistent with products from other sources.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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