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Goat Anti-Ku80 / XRCC5 Antibody - Trial (EB07209-T)

Code Name Applications Availability Size Tested species Price Grade  
EB07209-T Goat Anti-Ku80 / XRCC5 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

TestShot
EB07209-T 1
EB07209 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07209-T 2
EB07209 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: XRCC5, KU80, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining Ku autoantigen, 80kDa), HGNC:12833, KARP-1, KARP1, Ku86, NFIV, ATP-dependent DNA helicase II, DNA repair protein XRCC5, Ku86 autoantigen related protein 1, Ku autoantigen, 80kD, X-ray repair, complementing defective, repair in Chinese hamster
Official Symbol: XRCC5
Accession Number(s): NP_066964.1
Human GeneID(s): 7520

Immunogen

Peptide with sequence C-SLAKKDEKTDTLED, from the internal region of the protein sequence according to NP_066964.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 80-85kDa band observed in lysates of HeLa (calculated MW of 82.7kDa according to NP_066964.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of 75kDa was observed consistent with products from other sources.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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