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Goat Anti-LASP1 (internal region) Antibody (EB08165)

Related Products: EBP05251 - Immunizing Peptide, EBP08165 - Immunizing Peptide, Goat Anti-LASP1 (N Terminus) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08165 Goat Anti-LASP1 (internal region) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08165 1
EB08165 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08165 2
EB08165 (2.5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08165 3
EB08165 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: LASP1, LIM and SH3 protein 1, Lasp-1, MLN50
Official Symbol: LASP1
Accession Number(s): NP_006139.1
Human GeneID(s): 3927

Immunogen

Peptide with sequence C-PERRDSQDGSSYR, from the internal region of the protein sequence according to NP_006139.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 36kDa band observed in Human Kidney lysates (calculated MW of 29.7kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan 30;207(2):171-5; PMID: 9511759). Recommended concentration: 0.03-0.1µg/ml.
IHC: Paraffin embedded Human Colon and Small Intestine. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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