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Code Name Applications Availability Size Tested species Price Grade  
EB07682 Goat Anti-Latexin Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human, Mouse, Rat $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07682 1
EB07682 (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: latexin, LXN, ECI, TCI, endogenous carboxypeptidase inhibitor, MUM, tissue carboxypeptidase inhibitor
Official Symbol: LXN
Accession Number(s): NP_064554.2
Human GeneID(s): 56925
Non-Human GeneID(s): 17035 (mouse), 59073 (rat)

Immunogen

Peptide with sequence C-QYGTKVKHNSRLPKE, from the C Terminus of the protein sequence according to NP_064554.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28kDa band observed in lysates of Human Brain (Cerebellum), Mouse Brain and Rat Brain (calculated MW of 25.8kDa according to NP_064554.2). Recommended concentration: 0.3-1µg/ml.
IHC: This antibody has been successfully used in IHC-P for mouse bones as described in the following paper: Kadouchi et al, Biochem Biophys Res Commun. 2009 Jan 16;378(3):600-4; PMID: 19059214.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in the following paper:
Kadouchi I, Sakamoto K, Tangjiao L, Murakami T, Kobayashi E, Hoshino Y, Yamaguchi A.
Latexin is involved in bone morphogenetic protein-2-induced chondrocyte differentiation.
Biochem Biophys Res Commun. 2009 Jan 16;378(3):600-4.
PMID: 19059214

This antibody has been successfully used in the following paper:
Mitsunaga K, Kikuchi J, Wada T, Furukawa Y.
Latexin regulates the abundance of multiple cellular proteins in hematopoietic stem cells.
J Cell Physiol. 2011 May 12.
PMID: 21567403

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