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Goat Anti-LCK (aa39-52) Antibody (EB11387)

Related Products: EBP11387 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11387 Goat Anti-LCK (aa39-52) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat, Pig $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11387 1
EB11387 (0.1µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11387 2
EB11387 (0.3µg/ml) staining of Rat Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11387 3
EB11387 (1µg/ml) staining of Pig Bone Marrow (A) and Spleen (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: leukocyte C-terminal Src kinase, LSK, lymphocyte cell-specific protein-tyrosine kinase, lymphocyte-specific protein tyrosine kinase, OTTHUMP00000008640, OTTHUMP00000008740, OTTHUMP00000008741, p56(LSTRA) protein-tyrosine kinase, p56lck, pp58lck, proto-oncogene tyrosine-protein kinase LCK, t cell-specific protein-tyrosine kinase, T-lymphocyte specific protein tyrosine kinase p56lck, tyrosine-protein kinase Lck, YT16, LCK
Official Symbol: LCK
Accession Number(s): NP_005347.3
Human GeneID(s): 3932
Non-Human GeneID(s): 16818 (mouse), 313050 (rat)
Important Comments: This antibody is not expected to cross-react with other tyrosine protein kinases. Reported variants represent identical protein: NP_005347.3, NP_001036236.1

Immunogen

Peptide with sequence C-RNGSEVRDPLVTYE, from the internal region (near N Terminus) of the protein sequence according to NP_005347.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 60kDa band observed in Human and Rat Thymus lysates (calculated MW of 58.0kDa according to NP_005347.3). Recommended concentration: 0.1-0.3µg/ml. Approx 60kDa band observed in Pig Bone Marrow and Spleen lysates (calculated MW of 58.2kDa according to NP_001137185.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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