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Code Name Applications Availability Size Tested species Price Grade  
EB12395 Goat Anti-LDHB Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $423.00 Elite

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EB12395 1
EB12395 (0.1µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12395 2
EB12395 (0.1µg/ml) staining of Pig Heart lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Target Protein

Principal Names: LDHB, lactate dehydrogenase B, LDH-B, LDH-H, LDHBD, TRG-5, L-lactate dehydrogenase B chain, LDH heart subunit, lactate dehydrogenase H chain, renal carcinoma antigen NY-REN-46
Official Symbol: LDHB
Accession Number(s): NP_002291.1
Human GeneID(s): 3945
Non-Human GeneID(s): 16832 (mouse), 24534 (rat)
Important Comments: Reported variants represent identical protein: NP_002291.1, NP_001167568.1.

Immunogen

Peptide with sequence C-NARGLTSVINQKLK, from the internal region (near C terminus) of the protein sequence according to NP_002291.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 35kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 36.6kDa according to NP_002291.1). Recommended concentration: 0.03-0.1µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371