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Goat Anti-LDHC (aa 217 - 231) Antibody (EB07977)

Related Products: EBP07977 - Immunizing Peptide, EBP07978 - Immunizing Peptide, Goat Anti-LDHC (aa 221 - 233) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07977 Goat Anti-LDHC (aa 217 - 231) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07977 1
EB07977 (0.03µg/ml) staining of Mouse Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07977 2
EB07977 (1µg/ml) staining of paraffin embedded Mouse Testis. Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.
Click here to download PDF datasheet.

Target Protein

Principal Names: LDHC, MGC111073, lactate dehydrogenase C, LDH3, LDHX
Official Symbol: LDHC
Accession Number(s): NP_002292.1; NP_059144.1
Human GeneID(s): 3948
Non-Human GeneID(s): 16833 (mouse)
Important Comments: Both variants represent identical product (NP_002292.1 and NP_059144.1).

Immunogen

Peptide with sequence C-KLGTDSDKEHWKNIH, from the Internal region of the protein sequence according to NP_002292.1; NP_059144.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 30-35kDa band observed in Mouse Testis lysates (calculated MW of 36.6kDa according to human NP_002292.1 and 35.9kDa according to mouse NP_038608.1). Recommended concentration: 0.03-0.1µg/ml. This antibody has been successfully used in WB on Mouse, PMID: 36464740.
IHC: In paraffin embedded Mouse Testis shows strong signal in seminiferous tubules of Mouse Tesits. Recommended concentration, 1-2µg/ml. This antibody has been successfully used in IHC on Mouse, PMID: 36464740.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse

Specific Reference

This antibody has been successfully used in WB and IHC on Mouse:
Rie Iida-Norita, Haruhiko Miyata, Yuki Kaneda, Chihiro Emori, Taichi Noda, Tatsuya Nakagawa, Martin M Matzuk, Masahito Ikawa
Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs.
Andrology. 2023 Jul;11(5):840-848.
PMID: 36464740

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