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Goat Anti-Leptin Receptor Antibody - Trial (EB07263-T)

Related Products: EBP07263 - Immunizing Peptide, Goat Anti-Leptin Receptor Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07263-T Goat Anti-Leptin Receptor Antibody - Trial Pep-ELISA, WB, IHC, FC Yes 20µg specific antibody in 40µl Human, Mouse, Rat $105.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB07263-T 1
EB07263 (1.75µg/ml) staining of Human Cerebellum (A), and (1.5ug/ml) Mouse Foetal Brain (B) and Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07263-T 2
EB07263 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
EB07263-T 3
EB07263 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
EB07263-T 4
EB07263 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: LEPR, leptin receptor, CD295, OBR, OB receptor, LeprB
Official Symbol: LEPR
Accession Number(s): NP_002294.2; NP_001003679.1; NP_001003680.1
Human GeneID(s): 3953
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1).

Immunogen

Peptide with sequence C-TQDDIEKHQSDAG, from the internal region of the protein sequence according to NP_002294.2; NP_001003679.1; NP_001003680.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 140-150kDa band observed in Human Cerebellum, Mouse Foetal Brain and Rat Brain lysates (calculated MW of 132kDa according to Human NP_002294.2 and 130.8kDa according to Mouse NP_666258.2 and Rat NP_036728.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 4-6µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Dog

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