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Goat Anti-LIM domain only 3 / lmo3 Antibody (EB12770)

Related Products: EBP12770 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12770 Goat Anti-LIM domain only 3 / lmo3 Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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EB12770 1
EB12770 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB12770 2
EB12770 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: Lmo3, LIM domain only 3, AI854781, BB106490, Rbtn-3, Rbtn3, LIM domain only protein 3, LIM only 3, LMO-3, neuronal-specific transcription factor DAT1
Official Symbol: Lmo3
Accession Number(s): NP_061110.2; NP_001230540.1; NP_001230541.1; NP_001230542.1
Human GeneID(s): 55885
Non-Human GeneID(s): 109593 (mouse)

Immunogen

Peptide with sequence C-EGLMKEGYAPQVR, from the C Terminus of the protein sequence according to NP_061110.2; NP_001230540.1; NP_001230541.1; NP_001230542.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: This antibody has been successfully used in IHC on Mouse, PMID: 32562661.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog, Pig

Specific Reference

This antibody has been successfully used in IHC on Mouse:
Sara Brignani, Divya D A Raj, Ewoud R E Schmidt, Özge Düdükcü, Youri Adolfs, Anna A De Ruiter, Mateja Rybiczka-Tesulov, Marieke G Verhagen, Christiaan van der Meer, Mark H Broekhoven, Juan A Moreno-Bravo, Laurens M Grossouw, Emilie Dumontier, Jean-François Cloutier, Alain Chédotal, R Jeroen Pasterkamp
Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development.
Neuron. 2020 Aug 19;107(4):684-702.
PMID: 32562661

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