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Goat Anti-LIVIN / BIRC7 Antibody - Trial (EB05088-T)

Code Name Applications Availability Size Tested species Price Grade  
EB05088-T Goat Anti-LIVIN / BIRC7 Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

TestShot
EB05088-T 1
EB05088 staining (0.3µg/ml) of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05088-T 2
EB05088 staining (0.5µg/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BIRC7, LIVIN, baculoviral IAP repeat-containing 7 (livin), KIAP, MLIAP, mliap, ML-IAP, livin inhibitor-of-apotosis, kidney inhibitor of apoptosis protein, melanoma inhibitor of apoptosis protein, baculoviral IAP repeat-containing protein 7, RNF50, livin inhibitor of apoptosis
Official Symbol: BIRC7
Accession Number(s): NP_647478.1; NP_071444.1
Human GeneID(s): 79444
Important Comments: This antibody is expected to recognise both reported isoforms (as represented by NP_647478.1 and NP_071444.1)

Immunogen

Peptide with sequence C-RAPVRSRVRT, from the C Terminus of the protein sequence according to NP_647478.1; NP_071444.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 30kDa and 35kDa in lysates of cell line MOLT4, and 30kDa in lysates of Daudi, Jurkat and K562. These bands correspond to the reported isoforms with calculated MW of 32.8kDa according to NP_647478.1 and 30.9kDa according to NP_071444.1. Recommended concentration: 0.3 - 1 µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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