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Goat Anti-LMO7 / FBXO20 Antibody (EB06212)

Related Products: EBP06212 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06212 Goat Anti-LMO7 / FBXO20 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB06212 1
EB06212 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: LMO7, FBXO20, LOMP, FBX20, KIAA0858, LIM domain only 7, LOMP protein, F-box protein Fbx20, F-box only protein 20, zinc-finger domain-containing protein, RP11-332E3.2
Official Symbol: LMO7
Accession Number(s): AAD33924.1
Human GeneID(s): 4008
Important Comments: Please note that the amino acid sequence of the immuning peptide differs one amino acid from the current NCBI accession numbers (NP_005349.3, NP_056667.2). This antibody is expected to recognise both reported isoforms (NP_005349.3; NP_056667.2).

Immunogen

Peptide with sequence ESTCVSASLPRS, from the internal region of the protein sequence according to AAD33924.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70-75kDa band and approx 30kDa band observed in Human Lung lysates (predicted MWs of 92kDa according to AAD33924.1 - however our result agrees with Putilina et al as below). A minor band of unknown identity was also consistently observed at approx 50kDa. Recommended for use at 0.5-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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