Change country
Home

Goat Anti-LMP7 Antibody (EB09445)

Related Products: EBP09445 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09445 Goat Anti-LMP7 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09445 1
EB09445 (0.01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09445 2
EB09445 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PSMB8, proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7), D6S216, D6S216E, LMP7, MGC1491, PSMB5i, RING10, beta5i, OTTHUMP00000029420, OTTHUMP00000029421, OTTHUMP00000062981, low molecular weight protein 7, macropain subunit C13, multicatalytic endopeptidase complex subunit C13, protease component C13, proteasome (prosome, macropain) subunit beta type 8 (large multifunctional protease 7), proteasome beta 8 subunit, proteasome catalytic subunit 3i, proteasome subunit Y2, proteasome subunit beta 5i, proteasome-related gene 7
Official Symbol: PSMB8
Accession Number(s): NP_004150.1; NP_683720.2
Human GeneID(s): 5696
Non-Human GeneID(s): 16913 (mouse), 24968 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004150.1; NP_683720.2).

Immunogen

Peptide with sequence C-DVSDLLHQYREANQ, from the C Terminus of the protein sequence according to NP_004150.1; NP_683720.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 22kDa band observed in Human Liver, Lung and Lymph Node lysates (calculated MW of 29.8kDa according to precursor NP_004150.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schwarz et al, J Immunol. 2000 Jul 15;165(2):768-78. PMID: 10878350. Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Product Reviews

Please login to review this product.