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Goat Anti-LMX1B Antibody (EB08844)

Related Products: EBP08844 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08844 Goat Anti-LMX1B Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08844 1
EB08844 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: LMX1B, LIM homeobox transcription factor 1, beta, LMX1.2, MGC138325, MGC142051, NPS1, LIM homeo box transcription factor 1, beta
Official Symbol: LMX1B
Accession Number(s): NP_002307.1
Human GeneID(s): 4010
Non-Human GeneID(s): 16917 (mouse), 114501 (rat)

Immunogen

Peptide with sequence QQALTTSCYFRDRK , from the internal region of the protein sequence according to NP_002307.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 100kDa band in Mouse Kidney lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.6kDa according to Human NP_002307.1 and to Mouse NP_034855.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Testis. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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