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Goat Anti-LXR beta / NR1H2 Antibody (EB06521)

Code Name Applications Availability Size Tested species Price Grade  
EB06521 Goat Anti-LXR beta / NR1H2 Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06521 1
EB06521 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06521 2
EB06521 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06521 3
EB06521 (3µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB06521 4
EB06521 (3µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: NR1H2, LXR-b, NER, UNR, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor, LXRB, LX receptor beta, liver X receptor beta, liver X receptor-beta, nuclear orphan receptor LXR-beta, oxysterols receptor LXR-beta, steroid hormone-nuclear receptor NER
Official Symbol: NR1H2
Accession Number(s): NP_009052.3
Human GeneID(s): 7376
Important Comments: Based on the peptide used, this antibody is expected to be specific for LXR beta and not to cross-react with LXR alpha.

Immunogen

Peptide with sequence SSPTTSSLDTPLPGC, from the N Terminus of the protein sequence according to NP_009052.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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