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Goat Anti-LY6E (mouse) Antibody (EB10216)

Code Name Applications Availability Size Tested species Price Grade  
EB10216 Goat Anti-LY6E (mouse) Antibody Pep-ELISA, IF, FC, IHC Yes 100µg specific antibody in 200µl Mouse $315.00 Elite

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EB10216 1
EB10216 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10216 2
EB10216 Immunofluorescence analysis of paraformaldehyde fixed L929 cells (Mouse fibroblast cell line), permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10216 3
EB10216 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: lymphocyte antigen 6 complex, locus E, retinoic acid induced gene E, RIGE, RIG-E, SCA2, SCA-2, TSA-1, LY6E
Official Symbol: LY6E
Accession Number(s): NP_002337.1; NP_032555.1
Non-Human GeneID(s): 17069 (mouse)
Important Comments: Reported variants represent identical protein: NP_002337.1, NP_001120685.1

Immunogen

Peptide with sequence SCQEKDHYCIT, from the internal region of the protein sequence according to NP_002337.1; NP_032555.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments in Mouse Brain, Kidney, Heart and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: Customer obtained staining in Mouse Placenta in a pattern consistent with in situ hybridisation results, and no staining in KO mice. This antibody has been successfully used in IHC on Mouse: M. Hughes et al, Placenta. 2013 Sep;34(9):831-5. PMID: 23830620.

Immunofluorescence: Strong expression of the protein seen in the membranes of NIH3T3 and L929 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Mouse

Specific Reference

This antibody has been successfully used in IHC on Mouse:
M. Hughes, B.V. Natale, D.G. Simmons, D.R.C. Natale
Ly6e expression is restricted to syncytiotrophoblast cells of the mouse placenta
Placenta. 2013 Sep;34(9):831-5.
PMID: 23830620

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