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Goat Anti-MAD2L1 Antibody (EB05612)

Related Products: EBP05612 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05612 Goat Anti-MAD2L1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05612 1
EB05612 (2µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MAD2L1, MAD2 mitotic arrest deficient-like 1 (yeast), MAD2, HSMAD2, mitotic arrest deficient, yeast, homolog-like 1, MAD2 (mitotic arrest deficient, yeast, homolog)-like 1, MAD2-like 1, MAD2-like protein 1
Official Symbol: MAD2L1
Accession Number(s): NP_002349.1
Human GeneID(s): 4085
Non-Human GeneID(s): 56150 (mouse)

Immunogen

Peptide with sequence C-KVNSMVAYKIPVND, from the C Terminus of the protein sequence according to NP_002349.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 24kDa band observed in lysates of cell line HEK293 (calculated MW of 23.5kDa according to NP_002349.1). Recommended concentration: 2-4µg/ml. An additional band of unknown identity was also consistently observed at approx. 29kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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