Change country
Home

Goat Anti-MAOB Antibody (EB06984)

Related Products: EBP06984 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06984 Goat Anti-MAOB Antibody Pep-ELISA, WB, IF, IHC, FC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06984 1
EB06984 (0.1µg/ml) staining of Human Liver (A) and (0.3ug/ml) Testes (B) and Breast Cancer (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06984 2
EB06984 (0.1µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06984 3
EB06984 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06984 4
EB06984 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06984 5
EB06984 (5µg/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06984 6
EB06984 Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: MAOB, monoamine oxidase B, HGNC:6834, MGC26382, RP1-201D17__B.1, MAO, brain, MAO, platelet, adrenalin oxidase, amine oxidase (flavin-containing), tyramine oxidase
Official Symbol: MAOB
Accession Number(s): NP_000889.3
Human GeneID(s): 4129
Non-Human GeneID(s): 109731 (mouse)
Important Comments: This antibody is not expected to cross-react with MAOA.

Immunogen

Peptide with sequence C-HKARKLARLTKEE, from the internal region of the protein sequence according to NP_000889.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Human and Mouse Liver lysates and approx. 55-60kDa in Human Testis and Breast cancer lysates (calculated MW of 58.8kDa according to NP_000889.3). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Testis. Recommended concentration: 4-5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Product Reviews

Please login to review this product.