Change country
Home

Goat Anti-MAP3K6 Antibody (EB09554)

Code Name Applications Availability Size Tested species Price Grade  
EB09554 Goat Anti-MAP3K6 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB09554 1
EB09554 (0.3µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MAP3K6, mitogen-activated protein kinase kinase kinase 6, ASK2, MAPKKK6, MGC125653, MGC20114, OTTHUMP00000005063, apoptosis signal-regulating kinase 2
Official Symbol: MAP3K6
Accession Number(s): NP_004663.3
Human GeneID(s): 9064
Non-Human GeneID(s): 313022 (mouse), 53608 (rat)

Immunogen

Peptide with sequence C-RAILAQRAGSTP, from the C Terminus of the protein sequence according to NP_004663.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 140kDa band observed in Rat Heart lysates (calculated MW of 143kDa according to Human NP_004663.3 and Rat NP_001101379.1). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Product Reviews

Please login to review this product.