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Goat Anti-MARK4L Antibody (EB05383)

Related Products: EBP05383 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05383 Goat Anti-MARK4L Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05383 1
EB05383 (0.3µg/ml) staining of Human Cerebellum lysate (A) + peptide (B), Mouse Brain lysate (C) + peptide (D). (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05383 2
EB05383 (1µg/ml) staining of U251 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05383 3
EB05383 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: MARK4, MAP/microtubule affinity-regulating kinase 4, MARKL1, KIAA1860, Nbla00650, MARK4 serine/threonine protein kinase, MAP/microtubule affinity-regulating kinase like 1, FLJ90097, putative protein product of Nbla00650
Official Symbol: MARK4
Accession Number(s): NP_113605.2
Human GeneID(s): 57787
Important Comments: This antibody is expected to recognize the reported long isoform 1 (NP_113605.2) only.

Immunogen

Peptide with sequence IKRKPPPGCSDSPGV, from the C Terminus of the protein sequence according to NP_113605.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 70kDa band observed in Human Prostate lysates (calculated MW of 75.3kDa according to NP_113605.2). Recommended concentration: 0.3-1µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse

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