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Goat Anti-MBD2 (isoform 1) Antibody (EB07538)

Code Name Applications Availability Size Tested species Price Grade  
EB07538 Goat Anti-MBD2 (isoform 1) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $333.00 Elite

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EB07538 1
EB07538 (0.3µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07538 2
EB07538 (2.5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07538 3
EB07538 (2.5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07538 4
EB07538 (2.5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MBD2, methyl-CpG binding domain protein 2, DKFZp586O0821, DMTase, NY-CO-41
Official Symbol: MBD2
Accession Number(s): NP_003918.1
Human GeneID(s): 8932
Non-Human GeneID(s): 17191 (mouse)
Important Comments: This antibody is expected to resognize isoform 1 (NP_003918.1) only.

Immunogen

Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26(19):7224-35.; PMID: 16980624). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: 33283408 and 20523938.
IHC: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in WB on Human:
Cornelia G. Spruijt, Cathrin Grawe, Simone C. Kleinendorst, Marijke P. A. Baltissen and Michiel Vermeulen
Cross-linking mass spectrometry reveals the structural topology of peripheral NuRD subunits relative to the core complex
FEBS J. 2020 Dec 1. doi: 10.1111/febs.15650
PMID: 33283408

This antibody has been successfully used in WB on Human:
Spruijt CG, Bartels SJ, Brinkman AB, Tjeertes JV, Poser I, Stunnenberg HG, Vermeulen M.
CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.
Mol Biosyst. 2010 Sep;6(9):1700-6.
PMID: 20523938

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