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Code Name Applications Availability Size Tested species Price Grade  
EB10807 Goat Anti-Mboat4 (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10807 1
EB10807 (0.5µg/ml) staining of Mouse Pancreas lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Mboat4, membrane bound O-acyltransferase domain containing 4, GOAT, Gm171, O-acyltransferase (membrane bound) domain containing 4, O-acyltransferase domain-containing protein 4, ghrelin O-acyltransferase, membrane-bound O-acyltransferase domain-containing
Official Symbol: Mboat4
Accession Number(s): NP_001119786.1
Human GeneID(s): 619373
Non-Human GeneID(s): 234155 (mouse)

Immunogen

Peptide with sequence CHLGLHYTEYYLGEP, from the internal region of the protein sequence according to NP_001119786.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50kDa band observed in Mouse Pancreas lysates (calculated MW of 49.9kDa according to NP_001119786.1). Recommended concentration: 0.5-2µg/ml. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Pig, Cow

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