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Goat Anti-MDFI Antibody (EB09464)

Related Products: EBP09464 - Immunizing Peptide, EBP09950 - Immunizing Peptide, Goat Anti-MDFI Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09464 Goat Anti-MDFI Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09464 1
EB09464 (5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MDFI, MyoD family inhibitor, I-MF, I-mfa, OTTHUMP00000016375, OTTHUMP00000196414, inhibitor of MyoD family a
Official Symbol: MDFI
Accession Number(s): NP_005577.1
Human GeneID(s): 4188

Immunogen

Peptide with sequence C-SKSTTSQIPLQAQED, from the internal region of the protein sequence according to NP_005577.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx. 70kDa band in lysates of cell line HepG2 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25kDa according to NP_005577.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Lung (Bronchus). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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