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Goat Anti-MEIS2 Antibody (EB10049)

Related Products: EBP10049 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10049 Goat Anti-MEIS2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10049 1
EB10049 (0.1µg/ml) staining of Mouse Brain (lane A), Rat Brain (lane B), Mouse Thymus (lane C) and Rat Thymus (lane D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HsT18361, Meis homeobox 2, Meis homolog 2, Meis1, myeloid ecotropic viral integration site 1 homolog 2, Meis1-related gene 1, MGC2820, MRG1, OTTHUMP00000160209, TALE homeobox protein Meis2, MEIS2
Official Symbol: MEIS2
Accession Number(s): NP_733777.1; NP_733775.1; NP_758526.1
Human GeneID(s): 4212
Important Comments: This antibody is expected to recognize reported Human isoforms a (NP_733777.1), c (NP_733775.1), and g (NP_758526.1), and reported Mouse isoforms 3, 4 and 5 (NP_001153039.1, NP_001153040.1, NP_001153041.1). NB: The immunizing peptide shows no overlap with the reported PROVISIONAL Rat protein NP_001101228.1 but is does with UniprotKB entry D4A2T5.

Immunogen

Peptide with sequence C-QSNRAGFLLDPSVSQ, from the internal region of the protein sequence according to NP_733777.1; NP_733775.1; NP_758526.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50kDa bands observed in Rat Brain and Rat Thymus lysates, 48kDa band in Mouse Brain and 40kDa in Mouse Thymus (calculated MW of 51.8kDa according to Human NP_733775.1 and Mouse NP_001153040.1, and of 43.7kDa according to Mouse NP_001153039.1 and NP_001153041.1). Recommended concentration: 0.1-0.3 µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Human, Mouse, Dog, Cow

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