Change country
Home

Goat Anti-MEPI / SERPINI2 Antibody (EB06689)

Related Products: EBP06689 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06689 Goat Anti-MEPI / SERPINI2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06689 1
EB06689 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06689 2
EB06689 (3µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining
Click here to download PDF datasheet.

Target Protein

Principal Names: SERPINI2, MEPI, PI14, PANCPIN, TSA2004, SERPIN12, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2, serpin peptidase inhibitor, clade I (pancpin), member 2, myoepithelium-derived serine protease inhibitor, protease inhibitor 14, serine (or cysteine) proteinase inhibitor, clade I (pancpin), member 2
Official Symbol: SERPINI2
Accession Number(s): NP_006208.1; NP_001012303.1
Human GeneID(s): 5276
Non-Human GeneID(s): 67931 (mouse), 171149 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_006208.1; NP_001012303.1).

Immunogen

Peptide with sequence C-KGDWKQKFRKEDTQ, from the internal region of the protein sequence according to NP_006208.1; NP_001012303.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 40kDa band observed in Human Pancreas lysates (calculated MW of 46.1kDa according to NP_006208.1 and 47.4kDa according to NP_001012303.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This product has been successfully used in WB on Human in PMID: 21499210.
IHC: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cels and of rare cells in the islets of Langerhans. Recommended concentration, 3µg/ml. This product has been successfully used in IHC on Human in PMID: 21499210.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in WB and IHC on Human:
Wehr AY, Furth EE, Sangar V, Blair IA, Yu KH.
Analysis of the human pancreatic stellate cell secreted proteome.
Pancreas. 2011 May;40(4):557-66.
PMID: 21499210

Product Reviews

Please login to review this product.