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Code Name Applications Availability Size Tested species Price Grade  
EB06765 Goat Anti-MIF Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06765 1
EB06765 (0.01µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06765 2
EB06765 (0.01µg/ml) staining of Daudi (A) and Jurkat (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06765 3
EB06765 (5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), HGNC:7097, GIF, GLIF, MMIF, glycosylation-inhibiting factor, phenylpyruvate tautomerase, L-dopachrome isomerase, L-dopachrome tautomerase
Official Symbol: MIF
Accession Number(s): NP_002406.1
Human GeneID(s): 4282

Immunogen

Peptide with sequence C-NAANVGWNNSTFA, from the C Terminus of the protein sequence according to NP_002406.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 13kDa band observed in Human Lymph Node, and Thymus lysates and similar bands are observed in lysates of cell lines Daudi and Jurkat (calculated MW of 12.5kDa according to NP_002406.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Thymus. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Mouse

Product Reviews

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FAQs

What is the immunogen (antigen / peptide) sequence for this antibdoy?

The peptide sequence for this antibody is: NAANVGWNNSTFA.

 

In which species has the antibody been tested?

The antibody has been tested in human thymus and lymphnode lysates. Due to sequence similarities it may also work in mouse and rat tissues. Currently it is untested in mouse and rat tissues (please submit a product review).