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Goat Anti-MLC1 (aa179-193) Antibody (EB11998)

Related Products: EBP11998 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11998 Goat Anti-MLC1 (aa179-193) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11998 1
EB11998 (1µg/ml) staining of Human Hippocampus lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11998 2
EB11998 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MLC1, megalencephalic leukoencephalopathy with subcortical cysts 1, LVM, MLC, VL, membrane protein MLC1
Official Symbol: MLC1
Accession Number(s): NP_055981.1
Human GeneID(s): 23209
Important Comments: Reported variants represent identical protein: NP_631941.1, NP_055981.1.

Immunogen

Peptide with sequence C-SDSANILDEVPFPAR, from the internal region of the protein sequence according to NP_055981.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 70kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 41.1kDa according to NP_055981.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (López-Hernández et al, Hum Mol Genet. 2011 Aug 15;20(16):3266-77.PMID:21624973). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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